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PCR Fluorescence Analyzer Filters
The PCR fluorescence analyzer filter (e.g., real-time quantitative fluorescence PCR, qPCR) is the core component of the instrument and is responsible for selecting specific wavelengths of excitation and emission light for highly specific detection of fluorescent signals (e.g., dyes such as SYBR® Green, FAM, VIC, CY5, etc.).
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What are the characteristics of PCR fluorescence analyzer filters?
Main usage Fluorescence Microscope PCR Fluorescence Analyzer
Composition of membrane material Hard Coating
Production process High vacuum ion auxiliary coating technology
FWHM 10-50nm
Tpeak ≥80-90%
Blocking OD6
Conventional Wavelength 365mm,420mm,470nm,525mm,523mm,564mm,530mm,557nm,571nm,612mm,628nm,693mm

 Common fluorescent dyes and filters matching
Dye/Probe Excitation Wavelength Emission Wavelength Recommended Filter Combination
SYBR® Green 497 nm 520 nm 480–500 nm Ex / 510–540 nm Em
FAM 494 nm 520 nm 480–500 nm Ex / 510–540 nm Em
HEX/VIC 535 nm 556 nm 520–550 nm Ex / 550–580 nm Em
CY5 640 nm 670 nm 630–650 nm Ex / 660–690 nm Em
Texas Red 589 nm 615 nm 570–600 nm Ex / 610–630 nm Em
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